Fig. 3.

Comparison of microRNA (miR) and myogenic transcription factor Mef-2C mRNA levels in mouse C2C12 cells (reference) and HSkM. Under optimal conditions to promote C2C12 cell differentiation (8% serum and no Matrigel coating of plastic surface), expression of miR-1, miR-133a, and miR-206 was significantly higher in developing C2C12 myotubes. A–C: dynamic expression of miR-1, miR-133a, and miR-206 from day 0 to day 8 postdifferentiation. D: expression of Mef-2C was higher in C2C12 cell cultures than HSkM samples at all time points. Error bars, SE (n = 4). *P < 0.05 vs. HSkM.