Fig. 4.
Effect of PKC inhibitors GFI and Gö6983 on [Ca2+]e-evoked [Ca2+]i oscillations in CaRHEK-293 and SW-480 cells. A and B: CaRHEK-293 cells were treated with GF 109203X (GFI) at either 1 μM or 3.5 μM, GFV at 3.5 μM, and Gö6983 at 1 μM (Go) for 1 h, as indicated. Some cultures were treated with 100 nM phorbol-12,13-dibutyrate (PDBu) for 48 h. In all cases, cells were stimulated by raising [Ca2+]e to 3.0 mM. A: bars represent the percentage of cells showing [Ca2+]i oscillations. B: percentage of cells showing a nonoscillatory response. C and D: effect of PKC inhibitors GFI and Ro-31-8220 on [Ca2+]e-evoked [Ca2+]i oscillations in SW-480 cells expressing the CaR. SW-480 cells expressing the CaR were treated with the PKC inhibitors GF 109203X (GFI) at 3.5 μM or Ro-31-8220 (Ro) at 1 μM for 1 h before stimulation by raising [Ca2+]e to 3.0 mM. C: bars represent the percentage of cells showing [Ca2+]i oscillations. D: percentage of cells showing a nonoscillatory response. *Confidence intervals were not overlapping with control confidence intervals.