Fig. 5.
Inhibitors with selectivity for classic PKCs eliminated [Ca2+]e-evoked [Ca2+]i oscillations. CaRHEK-293 cells were treated for 1 h with the preferential inhibitors of classic PKCs Gö6976 (Go) and Ro-32-0432 (Ro) or inhibitors preferentially targeting PKC β, 539654 (539), Ly 3176155 (LY); an inhibitor for both classic and novel PKCs AEB071 (AEB), PKA (H-89), or PKD [kb NB 140–70 (kb)] at the indicated concentrations. Cells were then stimulated by raising [Ca2+]e to 3.0 mM. A: open bars show percentage of cells showing oscillations. B: closed bars show percentage of cells with nonoscillatory responses. *Confidence intervals were not overlapping with control confidence intervals.