Fig. 7.
Exposure to the calcimimetic R-568 induced [Ca2+]i oscillations in CaRHEK-293 cells. A: perfusion with saline containing R-568 at 50 nM was started at the upward arrow. R-568 was removed at the time marked by a downward arrow. B: perfusion with R-568 at 500 nM. C: percentage of cells oscillating, and the oscillation frequency are plotted at R-568 concentrations of 10, 50, 100, 200, and 500 nM. Data from 145 cells. D: response to 50 nM R-568 (marked by upward arrow) after pretreatment with the PKC inhibitor GFI (1 h, 3.5 μM). E: both PKC inhibitors Gö6983 (1 h, 1 μM) and GFI inhibited the oscillatory response to 50 nM R-568 (black bars) compared with control (white bar). Data are from 101 cells.