Fig. 1.

Representative Western blots of mitochondrial and nuclear OGG1 protein levels. Cells transduced with either an empty vector (Adv-Control) or a vector carrying the sequence of mitochondrial Ogg1 (Adv-Ogg1) were treated with menadione (50 μM) for 1 h. After menadione treatment, cells were washed with 1× PBS and incubated in fresh DMEM for 2 h at 37°C and 5% CO₂. Mitochondrial and nuclear proteins were isolated and used for Western blot analysis. The results demonstrated increased mitochondrial and nuclear Ogg1 expression in H9C2 cells and are representative of 3 independent experiments.