Fig. 2.

Markers of DNA damage and mRNA levels of the base excision repair (BER) pathway enzymes were measured in cells exposed to oxidative stress. Ogg1-transduced cells showed significantly reduced mitochondrial DNA (mtDNA) damage and increased expression of BER pathway enzymes. A: mitochondrial 8-OHdG levels normalized to total mtDNA. B: frequency of abasic (AP) sites in mtDNA. C and D: fold changes in the mRNA levels of APE1 and DNA γ polymerase compared with control. Bars represent means ± SE. Experiments were repeated 3 times, and assays were done in triplicate. *P < 0.001, **P < 0.005, #P < 0.0001.