Fig. 5.

Sidedness of EP4 receptor antagonism on PGE2-induced I_sc in mIMCD-K2 cells. A: representative traces show inhibition of I_sc^PGE2 when L-161,982 (2 × 10⁻⁸ M) is added to the apical side (dashed trace) or basal side (solid trace). B: average sustained I_sc after sequential addition of basal PGE2, 1 and 10 min after addition of L-161,982 (2 × 10⁻⁸ M). L-161,982 was added to either the apical bath (open bars) or basal bath (solid bars). Values are represented as means ± SE (n = 6 filters). *Values significantly different from that in cells treated with apical L-161,982.