Fig. 5.

IκKβ inhibition impairs IL-1β-mediated induction of the CXCL1 and CXCL2 genes. A and B: 832/13 cells were transfected with luciferase reporter constructs containing −1.5 kb of CXCL1 (A) or CXCL2 (B) promoters. The next day, cells were pretreated for 1 h with 2 μM TPCA and then stimulated with 1 ng/ml IL-1β for 4 h. Cells were lysed, and CXCL1 and CXCL2 promoter luciferase activity was quantified. #P < 0.1 vs. DMSO (A, B). C and D: 832/13 cells were pretreated for 1 h with 0.5, 1, or 2 μM TPCA, followed by 6 h stimulation with 1 ng/ml IL-1β. Relative CXCL1 and CXCL2 mRNA abundance was normalized to RS9. **P < 0.01 vs. DMSO (C, D). E and F: 832/13 cells were pretreated individually for 1 h with 10 μM of 3 different p38 inhibitors (SB202190, SB203580, and SB239063) followed by 6 h incubation with 1 ng/ml IL-1β. Relative mRNA abundance of CXCL1 and CXCL2 was calculated; n.s. vs. DMSO (E, F). Data shown represent means ± SE from 3 individual experiments.