Table 2.

Potential STAT1 binding sites within the rat CXCL1 and CXCL2 gene promoters

CXCL1	CXCL2	CXCL1/CXCL2
TTTGCAGAA	TTCTGAGTA	−1445/−1289
TTCAAGGAC	TTCTCTGTA	−887/−1258
TTCAGGAA	TTTCCTGAA	−653/−732
TTCTGGAA	TTCATGAA	−625/−723
TCCAGCGAA	TTCCCGAA	−218/−665
CTCCGGGAA*	TTCACAGAG	−87/−656
ND	TTCCTCAAA	−317
ND	TTCCCTGAT	−67

In silico examination of 1.5-kb regions controlling the proximal gene promoters for CXCL1 and CXCL2 reveals several regulatory sequences that are similar to a consensus γ-activated sequence (GAS) element. The putative STAT1 elements shown are either different by one nucleotide (nt) from a consensus GAS element or have a 2-nt spacer (NN) instead of a 3-nt spacer (NNN). One element in the CXCL1 gene promoter is a composite GAS/NF-κB sequence with demonstrated stimulus-induced STAT1 occupancy as shown in Fig. 9G.

^*Part of a Composite Element. Consensus GAS: TTCNNNGAA, where N is any nucleotide.