Table 3.
Enriched BAT proteomic pathways in k-means patterns of fall transition 13-lined ground squirrels determined from identified protein spots differing by Tukey HSD from either summer active ground squirrels or any heterothermic winter groups
| Annotation Term | Score | Fold | Genes |
|---|---|---|---|
| Fall Pathways Already in Winter Form (Decreased Abundance) | |||
| Mitochondrion | 7.1 | 6 | SDHA, PPP2R1A, UQCRC1, ACO2, GFM1, PITRM1, COQ9, SOD1, ACAT1, HIBADH, IDH3A, SOD2 |
| Aerobic respiration | 3.9 | 60 | SDHA, UQCRC1, ACO2, IDH3A |
| Complex assembly | 3.2 | 7 | PPP2R1A, GSN, FGB, FKBP4, GPX3, ANXA5, SOD2 |
| Organelle lumen | 2.5 | 3 | P4HB, CES1, ACO2, FGB, FKBP4, PITRM1, SOD1, ACAT1, IDH3A, SOD2 |
| Reactive O2 species response | 2.2 | 52 | GPX3, SOD1, SOD2 |
| Apoptosis regulation | 1.5 | 3 | PPP2R1A, SOD1, ANXA5, GSTP1, SOD2 |
| Extracellular space | 1.3 | 3 | GSN, FGB, GPX3, SOD1 |
| Fall Pathways in Already Winter Form (Increased Abundance) | |||
| Mitochondrial membrane | 11.5 | 16 | CPT2, SAMM50, IMMT, PHB, STOML2, UCP1, CRAT, OGDH, TIMM44, HADHA, HADHB, ACADVL, NDUFS8, ETFDH, MDH2 |
| Fatty acid β-oxidation | 8.0 | 121 | ACADVL, CPT2, ACADM, ETFDH, CRAT, HADHA, HADHB |
| TCA cycle | 4.1 | 39 | DLST, DLD, OGDH, IDH3A, MDH2 |
| Organelle lumen | 3.5 | 3 | DLST, ACADM, PHB, ERP29, OGDH, TIMM44, IDH3A, HADHA, HADHB, ACADVL, DLD, HSPA5, MDH2 |
| Electron carrier activity | 3.4 | 11 | ACADVL, ACADM, NDUFS8, DLD, ETFDH |
| Fatty acid binding | 1.9 | 39 | FABP3, HADHA, HADHB |
| Glucose catabolism | 1.8 | 25 | OGDH, MDH2, ENO1 |
Note: Enrichments determined by DAVID. “Score” denotes DAVID enrichment score (significance set >1.3). “Fold” denotes pathway fold enrichment. Redundant pathways and those contained as subsets within broader pathways were removed.