Table 4.

Enriched BAT proteomic pathways in k-means patterns of spring transition 13-lined ground squirrels determined from identified protein spots differing by Tukey HSD from either spring (SpC or SpW) ground squirrels, or any heterothermic winter groups

Annotation Term	Score	Fold	Genes
Spring Transition Pathways Already in Terminally Aroused Form (Decreased Abundance)
Coenzyme binding	7.1	26	ACADVL, ALDH6A1, ACADM, OGDH, HADH, IDH3A, HADHA, HADHB, ETFA
Mitochondrial membrane	6.3	12	ACADVL, COX7A2, CKMT1B, IMMT, PHB, HSPD1, OGDH, HADH, HADHA, HADHB
Organelle lumen	5.6	4	GRPEL1, ACADM, PHB, OGDH, IDH3A, HADHA, HADHB, ACADVL, LONP1, C1QBP, VCP, HSPD1, HSPA5, HADH, ETFA
Fatty acid binding	4.6	68	ALDH6A1, FABP3, FABP4, HADHA, HADHB
Fatty acid metabolism	4.3	55	ACADVL, ACADM, HADH, HADHA, HADHB
Adenyl nucleotide binding	3.4	4	ACADVL, TRAP1, LONP1, GRPEL1, ACADM, CKMT1B, VCP, HSPA5, HSPD1, SUCLA2, ETFA
Endopeptidase activity regulation	1.8	21	VCP, HSPA5, HSPD1
Spring Transition Pathways Already in Terminally Aroused Form (Increased Abundance)
Mitochondrion	3.7	6	GPD2, ACAA2, SHMT2, NDUFS8, ACAT1, IDH3A, PCCA
14-3-3	3.2	649	YWHAZ, YWHAB, YWHAQ, YWHAE
Complex assembly	2.8	7	PPP2R1A, FGB, FKBP4, NDUFS8, YWHAB, CDA
Phosphate metabolism regulation	1.8	6	PRKAR2B, PPP2R1A, YWHAB, FABP4, YWHAE
Cytoplasmic vesicle	1.7	5	YWHAZ, PRDX6, FGB, YWHAB, YWHAE
Apoptosis regulation	1.5	4	PPP2R1A, YWHAZ, YWHAB, YWHAE, GSTP1

Note: Enrichments determined by DAVID. “Score” denotes DAVID enrichment score (significance set >1.3). “Fold” denotes pathway fold enrichment. Redundant pathways and those contained as subsets within broader pathways were removed.