Table 5.
Omental WAT ECM parameters in healthy obese women and those with MetS
| Healthy Obese | MetS Obese | P Value | |
|---|---|---|---|
| Tensile strength, mN/mm2 | 34.7 ± 4.2 | 28.3 ± 4.4 | 0.31 |
| Sirius red (collagen) | 5,180 ± 1,880 | 5,404 ± 1,909 | 0.93 |
| DTAF (collagen/ECM) | 0.077 ± 0.010 | 0.073 ± 0.011 | 0.82 |
| Zymography (AU) | |||
| Pro-MMP-9 | 0.59 ± 0.13 | 0.70 ± 0.14 | 0.59 |
| Pro-MMP-2 | 0.99 ± 0.40 | 0.70 ± 0.14 | 0.52 |
| OmniMMP* | 667 ± 54 | 514 ± 113 | 0.21 |
| Gene expression** | |||
| COL1A2 | 100.0 ± 12.3 | 88.0 ± 12.2 | 0.50 |
| COL3A1 | 100.0 ± 13.1 | 80.0 ± 13.3 | 0.29 |
| COL5A2 | 100.0 ± 10.7 | 79.7 ± 13.9 | 0.25 |
| COL6A3 | 100.0 ± 12.2 | 64.0 ± 9.6 | 0.04 |
| COL8A1 | 100.0 ± 15.8 | 121.5 ± 37.9 | 0.58 |
| ELN | 100.0 ± 9.6 | 115.3 ± 22.2 | 0.51 |
| TNC | 100.0 ± 25.3 | 98.8 ± 21.8 | 0.97 |
| BGN | 100.0 ± 13.8 | 93.5 ± 16.8 | 0.77 |
| DCN | 100.0 ± 14.9 | 90.5 ± 9.7 | 0.62 |
| LUM | 100.0 ± 13.5 | 113.4 ± 19.8 | 0.57 |
| FBN1 | 100.0 ± 16.6 | 99.7 ± 21.6 | 0.99 |
| MFAP5 | 100.0 ± 21.1 | 113.4 ± 35.4 | 0.74 |
| MMP-2 | 100.0 ± 12.4 | 90.2 ± 18.4 | 0.65 |
| MMP-7 | 100.0 ± 59.9 | 112.6 ± 29.2 | 0.86 |
| MMP-9 | 100.0 ± 36.5 | 100.2 ± 25.4 | 0.99 |
| MMP-14 | 100.0 ± 11.2 | 92.9 ± 15.6 | 0.71 |
| CTSK | 100.0 ± 14.6 | 102.3 ± 12.3 | 0.91 |
| CTSL1 | 100.0 ± 14.5 | 104.9 ± 19.3 | 0.84 |
| CTSS | 100.0 ± 19.2 | 155.7 ± 43.8 | 0.23 |
| SERPINE1 | 100.0 ± 35.1 | 368.1 ± 113.6 | 0.02 |
| PLAUR | 100.0 ± 18.8 | 155.8 ± 26.6 | 0.09 |
Data are presented as means ± SE; n = 10 and n = 9 for healthy Sirius red and pro-matrix metalloproteinase-2 (MMP-2), respectively, and n = 9, 6, and 8 for MetS tensile strength, 5-(4,6-dichlorotriazinyl) aminofluorescein (5-DTAF), and pro-MMP-2, respectively. AU, arbitrary units; CTSK, cathepsin K; CTSL1, cathepsin L1; CTSS, cathepsin S;
OmniMMP units are emission at 405 nm minus blank.
mRNA abundances measured in a subset of subjects with available samples, expressed as percent of healthy obese group mean (n = 11 and 9 in healthy and MetS groups, respectively, for mRNA). Comparisons with P < 0.05 by Student's t-test are considered significantly different, and there was a trend for PLAUR (in boldface).