Table 2.
Biochemical parameters in the terminal blood sample
| n | PNa, mM | PK, mM | POsm, mOsm | Hematocrit, % | |
|---|---|---|---|---|---|
| HCTZ time course (clearance protocol A) | |||||
| Vehicle control | 5 | 146.6 ± 2.6 | 4.1 ± 0.4 | 303.8 ± 3.8 | 37.4 ± 1.7 |
| HCTZ (2 mg/kg) | 9 | 143.1 ± 2.8 | 4.1 ± 0.2 | 296.8 ± 5.6 | 40.3 ± 2.6 |
| HCTZ dose-range (clearance protocol C) | |||||
| Vehicle control | 9 | 146.4 ± 1.3 | 4.5 ± 0.6 | 304.0 ± 4.0 | 40.4 ± 1.2 |
| HCTZ (0.2 mg/kg) | 4 | 145.8 ± 1.7 | 4.8 ± 0.6 | 306.3 ± 8.0 | 38.5 ± 4.7 |
| HCTZ (2 mg/kg) | 9 | 143.1 ± 2.8 * | 4.1 ± 0.2 | 296.8 ± 5.6 | 40.3 ± 2.6 |
| HCTZ (20 mg/kg) | 8 | 143.6 ± 1.8 | 3.9 ± 0.3 | 296.9 ± 4.9 | 44.2 ± 1.3 * |
| HCTZ and BZM (clearance protocol D) | |||||
| BZM plus vehicle control | 6 | 142.2 ± 2.2 | 5.8 ± 0.2 | 306.0 ± 3.0 | 38.3 ± 3.2 |
| BZM plus HCTZ | 6 | 139.7 ± 3.9 | 5.5 ± 0.4 | 301.5 ± 5.8 | 43.2 ± 2.5 * |
Data are means ± 95% confidence intervals; n, no. of animals/group. PNa, concentration of Na+ in the plasma; PK, concentration of K+ in the plasma; POsm, plasma osmolality.
*
P < 0.05 for significant difference from the control group within each experiment by an unpaired t-test (clearance protocols A and D) or by a post hoc Dunnett's test after one-way ANOVA (clearance protocol C).