Fig. 9.

Glutamine synthetase (GS) expression in normal and acid-loaded diet C and CD-Rhbg/Rhcg-KO kidneys. A: immunoblot analysis of GS in the cortex and OM of CD-Rhbg/Rhcg-KO and C mice on a normal diet and when acid loaded. B: quantification of GS expression. CD-Rhbg/Rhcg-KO did not alter GS expression in either the cortex or OM in mice on a normal diet. In the cortex, acid-loading decreased GS expression in both genotypes, and GS expression was significantly decreased in acid-loaded CD-Rhbg/Rhcg-KO compared with acid-loaded C mice. Values are means ± SE; n = 4/group.