Fig. 6.

Serum creatinine and urea nitrogen levels are significantly decreased 24 h after bilateral IRI in mice treated with PD 0332991. A: serum creatinine levels increased to 2.27 ± 0.073 mg/dl in 8 vehicle-treated mice 24 h after bilateral IRI and 1.74 ± 0.143 mg/dl in 9 PD 0332991-treated mice. B: serum urea nitrogen levels in 6 vehicle-treated mice increased to 116.916 ± 2.74 mg/dl and 6 PD 0332991-treated mice had significantly lower urea nitrogen levels measured at 97.71 ± 4.31 mg/dl, 24 h after injury. C: tubular injury in mice that received vehicle or PD 033291. In vehicle-treated mice, outer medullary tubules are characterized by necrosis (*), hyaline casts, and evidence of epithelial damage in the majority of tubules. In the PD 033291-treated mouse kidney, damage was still present (*), but a substantial number of less damaged tubules was evident (arrowheads) where only mild tubular dilation is visible, without necrosis or cast formation. D: quantification of tubular injury score in vehicle (n = 6) or PD 033291 (n = 7) groups. Data analyzed by 2-way ANOVA followed by Bonferroni's posttest (A, B) or by 1-way ANOVA (D). ***P < 0.001, *P < 0.05.