Table 1.

Classification of sulfate esters employed as test substrates for the screening of whole microbial cells and/or purified enzymes for sulfatase activity

				Substrate	Type
	A		B		C		D		E
	R 1	R 2	R 1	R 2	R 1	R 2	R 1	R 2	R 1	R 2
1	H	n-C3H7	CH3	n-C3H7	CH=CH2	n-C4H9	CH3	C≡C–C2H5	CH3	3,5-(CF3)2C6H3
2	H	n-C4H9	CH3	n-C4H9	CH=CH2	n-C5H11	CH3	CH2–C≡C–CH3	CH3	p-F-C6H4-CH2
3	H	n-C5H11	CH3	n-C5H11	CH=CH2	n-C6H13	CH3	C≡C–Ph	CH3	p-Cl-C6H4-CH2
4	H	n-C6H13	CH3	n-C6H13	CH=CH2	n-C7H15	C2H5	C≡C–CH3
5	H	n-C7H15	CH3	n-C7H15	CH3	(CH2)2CH=C(CH3)2	C2H5	C≡C–C2H5
6	H	n-C8H17	CH3	n-C8H17	CH3	CH2–CH=CH2	C≡CH	n-C3H7
7	H	n-C9H19	CH3	n-C9H19	CH3	(CH2)2CH=CH2	C≡CH	CH2–CH(CH2)3
8	H	n-C11H23	CH3	n-C10H21			C≡CH	n-C4H9
9	H	n-C12H25	CH3	n-C12H25			C≡CH	n-C5H11
10	H	n-C13H27	C2H5	C2H5
11	H	n-C14H29	C2H5	n-C4H9
12	SLES		C2H5	n-C5H11
13	2-BOS		n-C3H7	n-C3H7
14			n-C3H7	n-C4H9
15			n-C3H7	n-C6H13
16			n-C4H9	n-C4H9
17			CH3	c-C6H11
18			CH3	Ph
19			CH3	CH2-Ph
20			CH3	(CH2)2-Ph

SLES sodium lauryl ether sulfate (Na⁺ n-C₁₂H₂₅–O–(CH₂)₂–O–SO₃ ⁻), 2-BOS 2-butyl-1-octyl sulfate