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AQP2 phosphorylation in isolated IMCD suspensions in the presence of vasopressin, tolvaptan dose-response. Tolvaptan/vehicle preincubation period was 30 min followed by addition of AVP at the indicated concentration or vehicle. Values are normalized band densities covering all bands (means ± SE); n = 4. *Statistical significance (P < 0.05, ANOVA with Bonferroni contrast).
