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Immunoblots of vasopressin dose-response experiments in isolated inner medullary collecting duct (IMCD) suspensions. Each lane represents a different vasopressin concentration (30-min incubation). Loading: AQP2, 5 μg; pS256, 20 μg; pS261, 20 μg; pS264, 15 μg; pS269, 20 μg; UT-A1, 30 μg; pS84, 30 μg; pS486, 30 μg; ERK 1/2, 20 μg; p38, 20 μg; JNK, 20 μg; NKCC1, 20 μg; and pNKCC1, 25 μg. Summary of all data is shown in Fig. 9.
