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. 2014 Feb 11;5:52. doi: 10.3389/fmicb.2014.00052

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Copyright © 2014 Bearson, Allen, Brunelle, Lee, Casjens and Stanton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Carbadox induction of Enterobacteriaeae prophages. Bacterial strains lysogenized with P22 (UB-1790), λ (UB-1703), HK97 (UB-1704), and Sf6 (UB-1496) were grown in LB broth at 37°C with shaking. At a density of 1 × 10⁸ bacterial cells/ml, carbadox was added to a final concentration of 0.5 μg/ml. Phage lysates were obtained by shaking with chloroform at the indicated times after carbadox addition and titered on a permissive host. Open symbols indicate cultures with no added carbadox and closed symbols indicate cultures with carbadox added. The different induced lysogens are indicated in the figure insert.