Fig. 1.

The downregulated in adenoma (DRA) 3′-untranslated region (UTR) sequence cloned into pmirGLO and the location of predicted microRNA (miRNA) seed sites. A: the DRA 3′-UTR and the location of predicted miRNA seed sites, mammalian conservation, and sequence alignment with relevant mammals. Broadly conserved miRNAs are conserved across most vertebrates. Placental Mammal Basewise Conservation is based on sequence alignment of 33 mammalian genomes, including those appearing individually below. The figure is based on the output from http://genome.ucsc.edu (hg19 assembly) and http://www.targetscan.org (B) miR-494 binding site on DRA 3′-UTR in various mammalian species using MEGA 5 software.