TABLE 2.
Effect of various treatments on brain-to-blood transport of methotrexate
| [3H]-Methotrexate | Concentration in the Injectate (mM) | Concentration in the Brain (mM) | BEI (%)a | Percentage of Control |
|---|---|---|---|---|
| WT (control) | — | — | 63.8 ± 5.2 | 100.0 |
| Mrp2−/− | — | — | 57.2 ± 6.0 | 90.0 |
| Bcrp1−/− | — | — | 46.4 ± 7.4b | 72.7 |
| WT + probenecid | 100 | 2.506 | 24.2 ± 8.6c | 37.9 |
| WT + benzylpenicillin | 100 | 2.506 | 13.7 ± 8.5c | 21.4 |
BEI, brain efflux index; WT, wild type.
a
Percentage of methotrexate eliminated from the ipsilateral cerebrum at 30 minutes.
b
P < 0.01.
c
P < 0.001.