Fig. 7.

Effect of phosphatidylinositol-3-OH kinase (PI3K)/Akt inhibition on the augmentation of CCh-induced contraction by BDNF. Administration of 10 μM LY294002 did not significantly inhibit contraction induced by 10 μM CCh. LY294002 introduced 15 min before and during BDNF incubation did not significantly reduce the augmentation of CCh-induced contraction by BDNF. Bar graph indicates CCh-induced contraction of muscle strips as a percentage of the control CCh-induced contraction obtained before treatment of muscle strips. Inset indicates representative immunoblot of phospho-Akt and GAPDH from muscle strip and from cultured muscle cells. Values are means ± SE. *P < 0.05, n = 5.