Fig. 4.

Chronic ethanol consumption increases sensitivity to Ca²⁺-mediated mitochondrial swelling. Isolated mitochondria were incubated in a KCl-based buffer and energized with oxidizable substrate. A: representative results of swelling in mitochondria incubated in 8 nmol Ca²⁺ and monitored at 540-nm absorbance. C: representative results of swelling in mitochondria from WT mice incubated in 40 nmol Ca²⁺ with and without 1 μM cyclosporin A (CsA) and monitored at 540-nm absorbance. E: representative results of swelling in mitochondria from CypD^−/− mice incubated in 40 nmol Ca²⁺ with and without 1 μM CsA and monitored at 540-nm absorbance. Decrease in absorbance was followed for 100–110 min. B, D, and F: summary statistics of onset (i.e., start time) of mitochondrial swelling for data in A, C, and E, respectively. Values are means ± SE; n = 4–6 mice for group (B, D, and F). Results for 2-factor ANOVA are presented in Table 2. B: ^aP < 0.05 vs. WT Con; ^bP < 0.05 vs. CypD^−/− Con; ^cP < 0.05 vs. WT EtOH. D: ^aP < 0.05 vs. WT Con; ^bP < 0.05 vs. WT EtOH. F: ^aP < 0.05 vs. CypD^−/− Con; ^bP < 0.05 vs. CypD^−/− Con + CsA.