Table 2.

Primers for V6 16S PCR

	Sequence (5′—3′)
Forward primer 1	[*]NCNACGCGAAGAACCTTANC
Forward primer 2	[*]NNCAACGCGAAAAACCTTACC
Forward primer 3	[*]NNNCAACGCGCAGAACCTTACC
Forward primer 4	[*]NNNNATACGCGARGAACCTTACC
Forward primer 5	[*]NNNNNCTAACCGANGAACCTYACC
Reverse primer	[†][6-bp index sequence][‡][4–7 N] CGACRRCCATGCANCACCT

^*Illumina TruSeq Universal Adapter sequence;

^†Illumina PCR primer;

^‡Illumina multiplexing PCR primer 2.0.