Fig. 1.

H₂O₂ levels increase in early points of pulmonary artery banding (PAB) in right ventricle (RV) but not in left ventricle (LV). Homogenates of RV and LV were incubated with Amplex Red solution, and fluorescence was measured for 1 h and 30 min at 37°C. H₂O₂ level is expressed as percentage of relative fluorescence units (RFU; arbitrary fluorescence units) of the respective sham for each time point. The RFU refers to the fluorescence measured in the linear part of the curve of H₂O₂ production. Results are expressed as means ± SE; n = 3–11 animals for the RV (A) and n = 3 animals for the LV (B). *P < 0.05 indicates significant difference vs. sham-operated animals.