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Nox4 protein expression increases in mitochondria after 6 h of PAB. Nox4 protein expression was measured by Western blot in isolated mitochondria from RV and normalized (Norm) to protein levels of mitochondria-specific cytochrome c oxidase I (COXI). A: representative blot. B: densitometry quantification of the blots. Data are expressed as means ± SE of fold change from sham; n = 4–8 animals. *P < 0.05 indicates significant difference vs. sham.
