Fig. 5.

Resveratrol decreases oxidative stress in cultured cerebromicrovascular endothelial cells and astrocytes. A: representative figures showing flow cytometric analysis of DCF fluorescence [indicating reactive oxygen species (ROS) production] in primary cerebromicrovascular endothelial cells (CMVECs) derived from young and aged F344xBN rats. Shown is the effect of resveratrol treatment (10 μmol/l, for 24 h) on ROS production by aged CMVECs. Appropriate control (unstained cells) is also shown. B: resveratrol (10 μmol/l, for 24 h) attenuates increased ROS production in aged CMVECs. Data are means ± SE (n = 8 in each group). *P < 0.05 vs. young control; ^#P < 0.05 vs. aged control. C: resveratrol (10 μmol/l, for 24 h) attenuates increased ROS production in activated astrocytes. Primary astrocytes in culture were stimulated by treatment with 10 ng/ml TNF-α, 10 ng/ml IL-1β, and 10 ng/ml IL-6 (for 4 h) with and without resveratrol pretreatment (10 μmol/l, for 24 h). Cellular ROS production was measured using the DCF fluorescence method by flow cytometry. Data are means ± SE (n = 8 in each group). *P < 0.05 vs. control; ^#P < 0.05 vs. stimulated cells (no resveratrol).