Fig. 8.

Cardiac function is impaired, but lateral fusion of myofibrils is not affected in cloche mutants. A and B: both heart rate and SF are reduced significantly in 52-hpf cloche mutants compared with control fish. *P < 0.01. C: the thick filament, thin filament, and Z-disc are revealed by immunostaining with anti-myosin antibody (Myosin), phalloidin (Actin), and anti-α-actinin antibody, respectively, in hearts isolated from 52-hpf cloche mutants and control fish. The lateral fusion of sarcomeres is intact in cloche mutants. Original scale bar, 5 μm.