Fig. 6.

A: effect of reversed blood flow on NADPH oxidase activity. Data are expressed as the ratio between reversed flow and forward flow (RF/FF), reversed flow + Jasp and forward flow (RF + Jasp/FF), and reversed flow and forward flow + apocynin (RF/FF + Apo) (left). Carotid arterial sections were immunostained with anti-4-hydroxynonenal (HNE) protein (green). Scale bar = 20 μm (right). B: effect of reversed blood flow vs. forward blood flow on NADPH oxidase protein expression (gp91^phox and p47^phox) determined by Western blot in samples untreated and treated with jasplakinolide. C: protein expression of gp91^phox and p47^phox in forward and reversed flow samples, untreated (left) and treated (right) with jasplakinolide. D: Western blot bands of gp91^phox and p47^phox from carotid vessels and white blood cells (WBC). DAPI, 4, 6-diamidino-2-phenylindole. *P < 0.05, RF/FF vs. FF/FF; **P < 0.05, RF + Jasp/FF vs. RF/FF; **^P < 0.05, RF/FF + Apo vs. RF/FF.