Fig. 5.

ASIC1 protein expression in small pulmonary arteries. Smooth muscle α-actin (SM α-actin) and ASIC1 immunofluorescence in ASIC1^+/+ and ASIC1^−/− lung sections demonstrate the presence of ASIC1 in small pulmonary arteries (∼70–100 μm). Fluorescence images were digitally inverted using ImageJ to provide better contrast and visibility of immunofluorescence. The bottom images represent digitally zoomed fluorescence overlay images corresponding to the boxes in the images of smooth muscle α-actin (green; top) and ASIC1 (red; middle).