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. 2014 Jan 20;10(2):171–180. doi: 10.7150/ijbs.7357

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Cerulenin-treatment inhibited migration and EMT of MCF-7-MEK5 cells. A. Wound healing assay. MCF-7-MEK5 confluent monolayers were scratched and an image was taken immediately (I). Serum-free medium only (II, control), medium with DMSO (III), or with Cerulenin (15 μg/ml, IV) were then added and incubated for 24 h. B. The migration index of the Cerulenin group was significantly lower than the DMSO and control group (p<0.01), suggesting that Cerulenin inhibited MCF-7-MEK5 cell migration. C. Cerulenin treatment increased E-cadherin mRNA and decreased vimentin mRNA in MCF-7-MEK5 cells. D. Quantitative real-time PCR assay showed that MCF-7-MEK5 cells had increased E-cadherin by 85.69±14.76 folds and decreased level of vimentin (0.016±0.004). E. WB assay showed an increase in E-cadherin and a decrease in vimentin proteins after Cerulenin treatment. *p<0.05, **p<0.01.