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. 2014 Jan 20;10(2):171–180. doi: 10.7150/ijbs.7357

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Immunofluorescence cytochemistry for localization of FASN with L-FABP or VEGF in MCF-7 and MCF-7-MEK5 cells. Localization of FASN (red) with VEGF (green) in MCF-7 cells (A) and MCF-7-MEK5 cells (B) were presented. DAPI staining was used to identify cell nuclei. Compared to the MCF-7 cells, MCF-7-MEK5 cells showed increased expression of FASN and VEGF and more co-labeling of two proteins (yellow) within cells except for nucleus. Localization of FASN (red) with L-FABP (green) in MCF-7 (C) and MCF-7-MEK5 cells (D) were presented. MCF-7-MEK5 cells showed higher level of L-FABP expression distributed in both cytoplasma and nucleus. Compared to the MCF-7 cells, MCF-7-MEK5 cells showed significant co-labeling of FASN and L-FABP (yellow) in most cell area except nucleus. Scale bar = 20 µm.