Skip to main content
. 2014 Feb 11;8:20. doi: 10.3389/fnins.2014.00020

Figure 1.

Figure 1

OXA hypothalamic fibers appose with SP positive DRG fibers in the dorsal horn. (A) Scheme of a spinal cord cross section (modified from OpenStax College PNS course). Dorsal horn, site of analyses presented below, is indicated in red. (B) Left panel: transversal section of spinal cord stained for SP. SP decorates lamina I and II of the dorsal horn. Right panel: longitudinal section of spinal cord stained for OXA. OXA is present in lamina I and II of the dorsal horn. (C) Transversal section of lamina I and II of the dorsal horn stained for OXA (red) and SP (green). (D) While some colocalization was visible in B, juxtaposition became clear only after a 3D reconstruction (SP in yellow and OXA in red). In inserts 1, 2, and 3, SP terminals in the section were colocalized or in contact with OXA terminals. Inserts were rotated by 180° (first panel under the insert) and by 270° (second panel under the insert). White circles show apposition of SP and OXA and a red circle shows an example of false positive.