Fig. 1.

Expression of lysyl hydroxylases during normal and aberrant late lung development. Expression levels of mRNA encoding plod1 (A), plod2 (B), and plod3 (C) were assessed by real-time RT-PCR in mRNA pools from lung homogenates from mouse pups at postnatal days (P) 2.5, 9.5, and 14.5, after exposure to 21% O₂ (●) or 85% O₂ (△) from P0.5. The 18S rRNA was used as a reference. Data reflect means ± SE (n = 5). The P values, indicated above pairs of data sets, were assessed by 1-way ANOVA with Tukey's post hoc test. D: protein expression of Plod1, Plod2, and Plod3 was assessed by immunoblot of protein extracts from lung homogenates from mouse pups over the course of late lung development, during exposure to 21% O₂ or 85% O₂ from P0.5. A single representative series is illustrated that is representative of at least 2 other series.