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. 2013 Nov 27;306(3):L246–L259. doi: 10.1152/ajplung.00109.2013

Fig. 5.

Fig. 5.

Regulation of lysyl hydroxylase mRNA levels by TGF-β. PLOD1, PLOD2, and PLOD3 expression was assessed by real-time RT-PCR using the primers listed in Table 1, in mRNA pools from human A549 cells (A), as well as in primary human lung fibroblasts (C), microvascular endothelial cells (E), and pulmonary artery smooth muscle cells (F) after stimulation with vehicle alone or TGF-β (2 ng/ml; 16 h). The HPRT gene was used as a reference. Similarly, plod1, plod2, and plod3 expression was assessed by real-time RT-PCR using the primers listed in Table 1, in mRNA pools from primary mouse lung alveolar type II cells (B), and mouse lung fibroblasts (D) after stimulation with vehicle alone or TGF-β (2 ng/ml; 16 h). The hprt gene was used as a reference. Data reflect means ± SD (n = 3). The P values (above the horizontal line) represented compare vehicle- vs. TGF-β-treated groups and were assessed by unpaired Student's t-test.