Fig. 8.

Impact of in vivo neutralization of TGF-β signaling on hyperoxia-induced lysyl hydroxylase expression. Expression levels of mRNA encoding plod1 (A), plod2 (B), and plod3 (C) were assessed by real-time RT-PCR in mRNA pools from lung homogenates from mouse pups at P10.5, after exposure to 21% O₂ (open bars) or 85% O₂ (closed bars) from P0.5 on, with concomitant administration of a pan-TGF-β-neutralizing antibody (1D11) or an isotype-matched control (MOPC21) antibody. The 18S rRNA was used as a reference. Data reflect means ± SD (n = 6, per group). The P values (presented below the horizontal brackets) were assessed by 1-way ANOVA with Tukey's post hoc test.