Fig. 4.

TNF-α-stimulated gene/protein 6 (TSG-6) dose response on SpO₂ and cellular infiltrates. A: effect of TSG-6 dose response on the dynamics of SpO₂ levels over the course of 8 days following bleomycin (Bleo) injury. A high dose (50 μg) and a low dose (12.5 μg) were administered on days 2 and 4 following bleomycin injury. B: dot plot represents the distribution of SpO₂ levels on day 8. Significant differences between high dose of TSG-6 and PBS groups was observed on day 8. For the analysis of cellular infiltrates, BALF was collected on days 2, 3, 5, and 8 following bleomycin injury. From day 3 onward following injury of the lungs with bleomycin, total cells (C) and macrophages (D) in BALF increased in the low-dose TSG-6 mice compared with PBS control mice. F and G: the high dose of TSG-6 lowered these parameters compared with PBS-treated mice with statistically significant differences between these 2 groups observed only on day 5. E and H: the low dose of TSG-6 increased neutrophil numbers early on day 2, whereas the high dose of TSG-6 maintained the neutrophil infiltration lower than PBS mice at all time points. Values are represented as arithmetic means ± SE (n = 5–8 mice per group). For the SpO₂ data, P value was calculated between PBS and TSG-6 groups by 1-tailed t-test. For the cellular infiltrates data, P values were calculated by 1-way ANOVA with a post hoc Bonferroni's multiple comparison analysis performed between all the groups. *P < 0.05, **P < 0.01.