Fig. 5.

TSG-6 treatment reduces cellular infiltration in the lungs during the early inflammatory phase. BALF was collected at 12 h and on days 2, 3, 4, 5, and 8 following bleomycin injury. On days 2 and 4 alone, BALF was collected 4 h after TSG-6 (50 μg) administration. From day 4 onward following injury of the lungs with bleomycin, total cells (A), macrophages (B), and neutrophils (C) in BALF increased compared with uninjured mice. TSG-6 treatment lowered these parameters compared with PBS-treated mice with statistically significant differences between these 2 groups observed only on day 5. Insets represent changes on day 5 in individual mice from the 3 groups. Note that on days 2 and 3 following bleomycin injury, macrophages (B) were significantly lower in PBS and TSG-6 groups (√√P < 0.01, √√√P < 0.001) compared with uninjured mice. Values are represented as arithmetic means ± SE (n = 5–8 mice per group). P values were calculated by 1-way ANOVA with a post hoc Bonferroni's multiple comparison analysis performed between all the groups. P values represented in the larger panels only indicate significant differences between PBS and TSG-6 groups following bleomycin injury. P values represented in the insets indicate significant differences between all 3 groups. *P < 0.05 and **P < 0.01.