Fig. 5.

Lung terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL): Ten fields per section from the regions with cell apoptosis were examined at a magnification of ×100. Lung sections from a saline-treated mouse (A) and LPS-treated (B) mouse did not show TUNEL-positive cells. Intravenous treatment with angiostatin of LPS-treated mouse (C) showed many apoptotic cells (arrows). An LPS-treated mouse given angiostatin subcutaneously (D) showed apoptosis, which appeared to be mostly in septal cells (40 ± 8.6/10 fields), whereas the intravenous angiostatin treatment group showed a significantly high number of TUNEL-positive cells in the parenchyma (bronchioles, septa, and blood vessels) (44 ± 3.7/10 fields) (E). ^a,bDifferent letters indicate statistical differences (P < 0.05) between the groups, while letters that match each other indicate no statistical difference.