Fig. 5.

Pulmonary bacterial clearance after 18 h of PAO1 infection. Data are presented as a scatterplot of lung homogenate colony-forming units (CFUs) from individual mice (⧫; n = 8–16 per group) 18 h after PAO1 infection (△ = average CFUs/lung ± SE). The dashed line represents an approximate clearance threshold, based on 3 times the observed standard deviation above the mean value in nondiabetic WT mice for this dose of bacteria. Lung homogenates that displayed less than 25,000 CFUs/lung were considered to be cleared of the instilled PAO1 bacteria. Most WT mice were cleared 18 h postinfection. Counts in nondiabetic WT mice were significantly lower than in nondiabetic CFKO mice. Cultures from diabetic WT mice had substantially increased CFUs/lung compared with nondiabetic WT mice. CFUs/lung from diabetic CFKO mice were also substantially increased compared with nondiabetic CFKO mice. Interestingly, lungs from diabetic CFKO mice had significantly increased numbers of PAO1 CFUs compared with lungs from diabetic WT mice, suggesting that diabetic CFKO mice have a diminished ability to properly clear PAO1 infections. *P < 0.05, **P < 0.01.