Fig. 3.
IL-17 and TNF-α enhance neutrophil infiltration and edema after I/R via NADPH oxidase. A: immunohistochemical staining of lung sections was performed to assess neutrophil infiltration after I/R in WT and p47phox−/− mice (p47−/−). Representative images of immunostaining are depicted wherein neutrophils are stained red, sections are counterstained with hematoxylin, and images are at ×40 magnification (bar = 50 μm). The number of neutrophils per high-power field (neutrophils/HPF) (B) and myeloperoxidase (MPO) levels in BAL fluid (C) were significantly increased after I/R in WT mice compared with sham, which were further enhanced by combined treatment with IL-17 and TNF-α. However, treatment of p47phox−/− mice undergoing lung I/R with IL-17 and TNF-α failed to enhance neutrophil numbers or MPO compared with untreated p47phox−/− mice. D: a significant increase in pulmonary edema (wet/dry weight) occurred after I/R in WT mice vs. sham, which was significantly enhanced after treatment with IL-17 and TNF-α. Edema was significantly attenuated in p47phox−/− mice compared with WT mice and remained unchanged after treatment with IL-17 and TNF-α. *P < 0.05 vs. WT sham; **P < 0.05 vs. all; #P < 0.05 vs. WT I/R; n = 5/group.