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. 2013 Dec 20;306(4):H585–H597. doi: 10.1152/ajpheart.00821.2013

Fig. 1.

Fig. 1.

Knockout of sirtuin 3 (Sirt3) reduces angiogenic growth factor expression. A: representative Western blot analysis of Sirt1 and Sirt3 expression. Sirt3 expression was absent in hearts of Sirt3KO mice (n = 5 mice; top). Sirt1 expression was not significantly difference in the hearts of Sirt3KO mice compared with wild-type (WT) mice (bottom; n = 3 mice; P > 0.05). NS, no statistic difference. B: representative images and Western blot analysis of angiopoietin-1 (Ang-1) expression. Ang-1 expression was significantly reduced in the hearts of Sirt3KO mice compared with WT control mice (n = 3 mice; *P < 0.05). C: Western blot analysis demonstrating that Ang-2 expression was significantly reduced in the hearts of Sirt3KO mice compared with WT control mice (n = 3 mice; *P < 0.05). D: representative Western blot analysis of Tie-2 in the hearts of WT and Sirt3KO mice. There was no significantly difference in Tie-2 expression between WT and Sirt3KO mice (n = 5–6 mice; P > 0.05). E: Western blot analysis demonstrating that the phosphorylation levels of Akt were significantly reduced in the hearts of Sirt3KO mice compared with WT control mice (n = 5–6 mice; *P < 0.05). F: Western blot analysis demonstrating that VEGFR2 expression was significantly decreased in the hearts of Sirt3KO mice compared with WT control mice (n = 3 mice; *P < 0.05). G: Western blot analysis demonstrating that VEGF expression was no significantly difference between WT and Sirt3KO mice (n = 5–6 mice; P > 0.05).