Fig. 2.

Preincubation with EETs prevents Aβ-induced mitochondrial depolarization. Astrocytes were loaded with EET after being treated with vehicle (A), Aβ for 24 h (B), Aβ with 12-h preincubation of 10 μM 14,15-EET (C), 10 μM 11,12-EET (D), or EETs alone (E and F) (×60 magnification, scale bar = 20 μm). The relative change in fluorescence intensity is expressed as percent change from vehicle set at 100% (G and H). Aβ causes a decrease in membrane potential compared with that of vehicle, which is reverted back to control levels after incubation with both EET regioisomers (G). EETs themselves do not affect the membrane potential (H). *P < 0.05 vs. vehicle; #P < 0.01 vs. Aβ; n = 5 to 6.