Fig. 1.

Characterization of immunoassays. A: sensitivity of an immunoassay originally optimized using mouse cardiac myosin binding protein-C (cMyBP-C) standards was tested using porcine and human standard curves of 40-kDa recombinant protein. Chemiluminescent signal was plotted against log cMyBP-C concentration. Dashed lines indicate the lowest signal detectable above blank. B: coefficient of variance and recovery of the assay (C) was plotted against the log cMyBP-C concentration. D: cardiac troponin I (cTnI), cardiac troponin T (cTnT), and MYL3 detection was performed in multiplex immunoassay with high sensitivity. Dashed lines indicate the lowest signal detectable above blank for each assay. Data are presented as means ± SE. Au, arbitrary units.