Figure 4.

Hit compounds identified from the high-content screen of small chemical library. The hit compounds (A–C) that induced a reproducible and significant increase of the granule average intensity (GAI) in U373-RTA_E177Qegfp cells are indicated by their structure, name, and average Robust Z score. The standard deviation value is from three replicates. Two chemically distinct groups of compounds comprised of acetyl isogambogic acid (AIGA), gambogic acid amide (GAA), and dihydrogambogic acid (DGA) (A) and dihydrocelastryl diacetate (DC) and celastrol (CEL) (B) were identified as hit compounds. Other hit compounds (C) include gentian violet (GV), anthothecol (ANTHO), tetrachloroisophthalonitrile (TCIN) and 1-benzyloxycarbonylaminophenethylchloromethyl ketone (BCPK).