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. 2013 Dec 23;6(1):33–53. doi: 10.3390/toxins6010033

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Stabilization of RTA_E177D by hit compounds. U373_RTAE177D cells treated with DMSO, ZL₃VS (3 µM) or hit compounds (2.5, 5, and 10 µM) were subjected to immunoblot analysis for RTA_E177D (A–C, lanes 1–9; D, lanes 1–12) and GAPDH (A–C, lanes 10–18; D, lanes 13–24). We analyzed the compounds anthothecol (ANTHO), acetyl isogambogic acid (AIGA) (A); celastrol (CEL), dihydrocelastryl diacetate (DC) (B); 1-benzyloxycarbonylaminophenethylchloromethyl ketone (BCPK), tetrachloroisophthalonitrile (TCIN) (C); gentian violet, merbromin (MB), and acriflavinium hydrochloride (AFH) (D). RTA polypeptides, GAPDH and molecular weight markers are indicated.