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. Author manuscript; available in PMC: 2014 Nov 5.
Published in final edited form as: Curr Protoc Microbiol. 2013 Nov 5;31:15K.1.1–15K.1.45. doi: 10.1002/9780471729259.mc15k01s31

Figure 2. VEE 3526 replicon norovirus capsid expression construct.

Figure 2

The capsid and E glycoprotein genes from Venezuelan equine encephalitis are replaced with the norovirus major capsid protein gene VP1. The VEE capsid and E glycoproteins are supplied in separate constructs. When cells are transfected with all three constructs, VEE replicons expressing norovirus VP1 are formed. These replicons can go through one round of replication, producing ample amounts of VP1 which subsequently self-assembles into VLPs.