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. 2014 Feb 11;5:38. doi: 10.3389/fphys.2014.00038

Figure 4.

Figure 4

Studying the effects of FGF2 on cellular calcium in satellite cells associated with host FDB fibers. Muscle cultures pre-stained with anti-CD34-FITC to identify satellite cells (A,C) were then loaded with calcium indicator X rhod-1 AM (E). Transmitted light images are shown in (B,D). Application of FGF2 at 2 ng/ml to the culture triggered a rise of calcium in the satellite cell in the confocal image plane (F; 9 satellite cells from 2 mice). Rinse with Ringer's solution without FGF2 reverses the rise in cell calcium (G; 9 cells from 2 mice). In cultures pre-loaded with calcium chelator BAPTA AM FGF2 caused little increase in calcium (H; 8 cells from 2 mice). In cultures rinsed with calcium free Ringer's solution, the effects of FGF2 are minimal (I; 8 cells from 2 mice). Incubation with TRPC blocker SKF 96365 attenuated the effects of FGF2 on cellular calcium (J; 8 satellite cells from 2 mice). FGF2 has no effects on cellular calcium of FDB fibers (K; 11 FDB fibers from 2 mice). **p < 0.01, compared to 0 min.