Figure 5.

Effects of FGF2 on satellite cell activation and proliferation. Freshly isolated FDB fibers associated with satellite cells were cultured for 24 h without FGF2, with FGF2, or with both SKF 96365 and FGF2. FGF2 significantly increased the number of MyoD+ cells associated with FDB fibers (middle column in A,B). In cultures treated with both SKF 96365 and FGF2, the induction of MyoD+ cells by FGF2 was partly diminished (right column in A,B). Data were from 17 satellite cells of 16 FDB fibers, 37 satellite cells of 14 FDB fibers, and 14 satellite cells of 10 FDB fibers from 3 mice, for left, middle, and right column respectively. ^**p < 0.01 compared to control.