Figure 3. Improving lectin binding using multimerization.

a) Lectins are pre-incubated with dye-labeled streptavidin to form multi-lectin units. b) After samples are incubated on antibody arrays, the capture proteins are probed with the multimers (top), in contrast to the standard protocol in which the lectins are not linked (bottom). c) Signals can be improved for certain assays, resulting in d) lower detection limits as shown by dilution curves for the detection of the protein TSP-5 by the lectin AAL.